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Real time monitoring of gene electrotransfer efficiency using different electroporation protocols

STSM by Urška Kamenšek, Early-Stage Researcher

Period of mission: from 02/03/2015 to 29/05/2015

Home institution: Institute of Oncology Ljubljana, Ljubljana, Slovenia

Host institution: Institute of Pharmacology and Structural Biology, Toulouse, France

Gene electrotransfer (GET) is becoming increasingly more recognized method for cancer gene therapy. Although, the method is generally safe, efficient, and can produce good reproducibility and spatial precision, the effective delivery of therapeutic gene to target tissue remains the stumbling block to its effective and broader use. Gene electrotransfer to tumors represent a special challenge due to the heterogeneity of tumors, low transfection efficiency and activation of non-specific antitumor effectiveness, most probably mediated by the induction of immune response. Therefore the purpose of my STSM was to further explore the phenomena associated with gene electrotransfer to tumors with real time evaluation of transfection efficiency as well as cell death following the treatment with noninvasive luminescence monitoring of the effects. Using the light producing tumors, the luminescence technology enabled us the in vivo monitoring of tumor growth, as well as the emergence of hypoxic - necrotic tumor center. Additionally, we were able to visualize the transient transfection after the i.t. GET of the luciferase expressing plasmid. The data obtained during the STSM provided a new insight on some of the phenomena’s associated with gene electrotransfer to tumors, like the vascular lock, the generation of the non-specific antitumor effectiveness and the localization of the source of expression. These can all have an impact on GET efficiency and have to be taken into account when designing improved and more effective cancer gene therapy, allowing the quicker translation of the technology into clinical trials.

Publication(s) originating from this mission

Journal Current Gene Therapy
Impact Factor (according to ISI Thomson) 2,542
Authors Urska Kamensek, Marie-Pierre Rols, Maja Cemazar, Muriel Golzio
Title Visualization of Nonspecific Antitumor Effectiveness and Vascular Effects of Gene Electro-Transfer to Tumors
Year 2015
Issue ?
Pages ?
Status Submitted - to be updated

 


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