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Implementation of an optical tweezers based system for the micromanipulation of electroporated cells

STSM by Tudor Savopol, Researcher

Period of mission: from 09/11/2014 to 15/11/2014

Home institution: Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Host institution: Institute of Pharmacology and Structural Biology (IPBS), CNRS, Toulouse, France

The main purpose of the STSM mission was to start developing a method, based on optical tweezers, which should permit to monitor modifications of optical properties of porated cells with a good time resolution (as compared to cellular events) subsequent to the electroporation. This can be done by measuring the force constant of a trapped cell before electroporation and at different moments after electroporation. In order to do this and to get quantitative robust results, the optical trap should be very well calibrated. The main problem in using optical traps is the calibration of its force. The purpose of the scientific work carried out during STSM was to setup a calibration method which can overcome these disadvantages. The proposed method is based on the use, in the optical path of the laser, before entering in the microscope, of a mirror mounted in the back focal plane of the first telescope of the tweezers, which allows a fast deflection with small well controlled angles, of the laser beam. We tested the method using beads of different materials and diameters, suspended in media with different viscosity. The results were consistent with the characteristics of the beads and proved to be very reproducible. Data collection protocols were established in order to optimize the method and to make it compatible with simultaneous use in electroporation experiments.

Poster presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period

06/09 - 10/09/2015

Authors Violeta Liuba Calin, Mihaela Georgeta Moisescu, Irina Grigorescu, Liviu Dimitriu and Tudor Savopol
Title Investigation of optical and mechanical characteristics of electroporated cells using optical tweezers

 


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