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Fast electrical bioimpedance spectroscopy measurements during electroporation

STSM by Tomás García-Sánchez, PhD Student

Period of mission: from 30/09/2013 to 01/11/2013

Home institution: Technical University of Catalonia (UPC), Electronic and Biomedical Instrumentation Group, Barcelona, Spain

Host institution: Laboratory of Vectorology and Anticancer Therapeutics, University Paris-Sud and Gustave Roussy, Paris, France

The study of the dynamics of cell membrane during electropermeabilization is one of the main topics in today’s electroporation research field. Accurate study of pore evolution over time is useful to extract information about the successfulness of electroporation treatment and cell recovering as well as the dynamics of these events. Measurement of the temporal evolution of the electrical impedance spectrum is a label-free technique that can provide valuable information about the state of the system. In this STSM a fast measuring technique (in the range of millisecond) was applied to online monitor the impedance spectra evolution from 5 kHz to 1.31 MHz during electroporation of cell monolayers. Also, as a proof of concept, some in vivo experiences were performed in skin electroporation using the same technique. The goal was to correlate the observed impedance changes with the cell permeabilization detected using a fluorescent dye. The results obtained show promising information on how the study of the complete spectral information can provide additional information about the system. The different effects occurring during the application of pulses such as temperature changes, reactions in the electrode/electrolyte interface, and changes in the conductivity of the extracellular medium due to ion efflux could be differentiated and characterized given the fact that their frequency response is different. Further work will comprise development of theoretical models to adjust the measurements obtained.

Publication(s) originating from this mission

Journal Bioelectrochemistry
Impact Factor (according to ISI Thomson) 4,172
Authors Tomás García-Sánchez, Antoine Azan, Isabelle Leray, Javier Rosell-Ferrer, Ramon Bragós, Lluis M. Mir
Title Interpulse multifrequency electrical impedance measurements during electroporation of adherent differentiated myotubes
Year 2015
Issue 105
Pages 123-135
Status Published

Oral presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period 06/09 - 10/09/2015
Authors Tomas Garcia-Sanchez, Antoine Azan, Isabelle Leray, Javier Rosell-Ferrer, Lluis M. Mir and Ramon Bragos
Title Modeling dynamic electrical impedance spectroscopy measurements on electroporated cells

 


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