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Fast electrical bioimpedance spectroscopy measurements during electroporation (part II)

STSM by Tomás García-Sánchez, PhD Student

Period of mission: from 19/05/2014 to 30/05/2014

Home institution: Technical University of Catalonia (UPC), Electronic and Biomedical Instrumentation Group, Barcelona, Spain

Host institution: Laboratory of Vectorology and Anti-cancer Therapeutics, UMR 8203 CNRS, University Paris Sud and Gustave Roussy, Paris, France

The activity in this STMS was motivated by previous work developed during the first visit to the Institute Gustave Roussy (Paris, France) for five weeks in October 2013. After deep analysis of the results of the first set of experiments we found that some new tests were necessary to improve the understanding of our previous findings. The study of the dynamics of cell permeabilization by means of electrical impedance spectroscopy measurements was extended to new cell lines. In total, five different cell lines were subjected to complete analysis. Electrical impedance of cell monolayers was monitored as a function of field magnitude within a classical electroporation treatment (8 pulses, 100 µs, 1 Hz). Measurements were extended during several minutes before and after pulse application. Additionally the study was repeated using different pulse repetition frequencies. The preliminary analysis of the data shows promising information about the different spectral behavior of the different phenomena occurring during pulse application. This opportunity was useful not only to confirm the analysis made from the previous STSM, but also to provide valuable and novel information. Further work will be focused on the detailed analysis of the very large amount of data collected during the STSMs to generate valuable information for publication.

Publication(s) originating from this mission

Journal Bioelectrochemistry
Impact Factor (according to ISI Thomson) 4,172
Authors Tomás García-Sánchez, Antoine Azan, Isabelle Leray, Javier Rosell-Ferrer, Ramon Bragós, Lluis M. Mir
Title Interpulse multifrequency electrical impedance measurements during electroporation of adherent differentiated myotubes
Year 2015
Issue 105
Pages 123-135
Status Published

Oral presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period 06/09 - 10/09/2015
Authors Tomas Garcia-Sanchez, Antoine Azan, Isabelle Leray, Javier Rosell-Ferrer, Lluis M. Mir and Ramon Bragos
Title Modeling dynamic electrical impedance spectroscopy measurements on electroporated cells

 


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