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Calcium electroporation on 3D multicellular spheroids

STSM by Stine Krog Frandsen, PhD Student

Period of mission: from 18/11/2013 to 13/12/2013

Home institution: Department of Oncology, Copenhagen University Hospital, Herlev, Denmark

Host institution: IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse, France

IPBS, Toulouse has experience in working with spheroid models and the purpose of the STSM was to perform calcium electroporation on 3D multicellular spheroids. This enables us to further investigate calcium electroporation in vitro in a tumor-like model and maybe in future experiments understand more about the mechanism behind calcium electroporation. To test the effect of calcium electroporation and to compare the treatment with electrochemotherapy we treated 4 different spheroids (SW780, human bladder cancer; HT29, human colon cancer; MDA-MB231, human breast cancer; HDF-n, human normal fibroblast). Size measurements (light microscope images) of each spheroid before and after treatment were performed as a measure of living/dead cells. The cancer cell spheroids treated with either calcium electroporation or bleomycin electroporation decrease in size after treatment whereas, interestingly, spheroids made of the normal fibroblast cell line (HDF-n) were not affected by any of the treatments and all spheroids grew similar to untreated controls. We also measured the intracellular ATP level in spheroids 1, 4, and 24 hours after treatment with calcium electroporation. The results indicate that the intracellular ATP decrease dramatically after treatment with calcium electroporation, in all 4 different spheroids, even in the fibroblast spheroids which are still living after treatment with calcium electroporation. Altogether, the experiments performed during the STSM confirm the results seen previously in vitro and in vivo, and the results of the spheroids made of normal fibroblasts are very interesting and gives us new knowledge in this research area.

Publication(s) originating from this mission

Journal Plos One
Impact Factor (according to ISI Thomson) 3,234
Authors Emilie L. Hansen, Esin B. Sozer, Stefania Romeo, Stine K. Frandsen, Paul T. Vernier, Julie Gehl
Title Dose-dependent ATP depletion and Cancer Cell Death following Calcium Electroporation, Relative Effect of Calcium Concentration and Electric Field Strength
Year 2015
Issue 10 (4)
Pages e0122973
Status Published

 

Journal ?
Impact Factor (according to ISI Thomson) ?
Authors Stine Krog Frandsen, Laure Gibot, Moinecha Madi, Julie Gehl, Marie-Pierre Rols
Title Calcium electroporation: Evidence for differential effects in normal and malignant cell lines, evaluated in a 3D spheroid model
Year ?
Issue ?
Pages ?
Status Under Review - to be updated

Conference abstract(s) originating from this mission

Publication IFMBE Proceedings of the 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Publisher Tomaz J, Kramar P (ed), 1st edn. Springer-Verlag, Singapur
Authors Stefania Romeo, Emilie L. Hansen, Stine K. Frandsen, Julie Gehl
Title Electroporation of a Bladder Cancer Cell Line in Presence of Calcium: Efficacy Dependence on Electric Field Strength and Calcium Concentration
Year 2015
Issue 53
Pages 151-154
Status Published

Oral presentation(s) originating from this mission

Event Bioelectrics 2014
Location Columbia/Missouri/USA
Period 12/10 - 18/10/2014
Authors Stine Krog Frandsen, Laure Gibot, Marie-Pierre Rols, Julie Gehl
Title Calcium electroporation of spheroids induce cell death in cancer cells, not normal cells

 

Event Gordon Research Conference in Bioelectrics
Location Maine/USA
Period 06/07 - 11/07/2014
Authors Stine Krog Frandsen, Laure Gibot, Marie-Pierre Rols, Julie Gehl
Title Calcium electroporation of spheroids induce cell death in cancer cells, not normal cells

 

Event Gordon Research Seminar in Bioelectrics
Location Columbia/Missouri/USA
Period 05/07 - 06/07/2014
Authors Stine Krog Frandsen, Laure Gibot, Marie-Pierre Rols, Julie Gehl
Title Calcium electroporation of spheroids induce cell death in cancer cells, not normal cells

 

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period 06/09 - 10/09/2015
Authors Stefania Romeo, Emilie L. Hansen, Stine K. Frandsen, Julie Gehl
Title Electroporation of a Bladder Cancer Cell Line in Presence of Calcium: Efficacy Dependence on Electric Field Strength and Calcium Concentration

Poster presentation(s) originating from this mission

Event 13th International Meeting of the European Calcium Society
Location Centre de Congrès/ Aix-en-Provence/Frankrig/France
Period

13/09 - 17/09/2014

Authors Stine Krog Frandsen, Laure Gibot, Marie-Pierre Rols, Julie Gehl
Title Calcium electroporation of spheroids induce cell death in cancer cells, not normal cells

 

Event Gordon Research Conference in Bioelectrics
Location Maine/USA
Period

06/07 - 11/07/2014

Authors Stine Krog Frandsen, Laure Gibot, Marie-Pierre Rols, Julie Gehl
Title Calcium electroporation of spheroids induce cell death in cancer cells, not normal cells

 


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