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Modeling of electroporation in presence of calcium

STSM by Stefania Romeo, Early-Stage Researcher

Period of mission: from 04/05/2014 to 27/05/2014

Home institution: Institute for Electromagnetic Sensing of the Environment (IREA) – National Research Council of Italy, Naples, Italy

Host institution: Department of Oncology, Copenhagen University Hospital, Herlev, Denmark

Electroporation in presence of calcium has been recently demonstrated by the host research group to be capable of inducing cell death, suggesting that calcium might be used as an alternative to bleomycin in the palliative treatment of tumors via electrochemotherapy. The first clinical trial using calcium electroporation has recently opened at Herlev Hospital, however better understanding of the mechanism behind calcium electroporation is highly warranted to optimize the effect. The activity carried out during the STSM consisted both in experimental studies on calcium electroporation and in a post-processing analysis of experimental data aimed to define mathematical relations between the experimental parameters. In particular, SW780 bladder cancer cells were exposed to 8, 99 µs long, 1 Hz repetition rate pulses with variable electric field (0.2 to 1.6 kV/cm) in presence of calcium (0, 1, 3, 5 mM), and cell viability was evaluated by means of the MTS assay at 1, 4, 8, 22 and 24 hours after electroporation. A mathematical fitting analysis of the experimental data was then carried out to estimate the electric field amplitudes leading to a desired percentage of cell death at different calcium concentrations, and to assess the dynamics of cell death under different electric fields and calcium concentration conditions. The procedure set up confirm previous in vitro results on calcium electroporation, can be tailored to different cell types to identify the optimum combinations of electrical conditions and calcium concentrations that maximize cell killing, and can also provide information to optimize application of calcium electroporation in vivo.

Publication(s) originating from this mission

Journal Plos One
Impact Factor (according to ISI Thomson) 3,234
Authors Emilie L. Hansen, Esin B. Sozer, Stefania Romeo, Stine K. Frandsen, Paul T. Vernier, Julie Gehl
Title Dose-dependent ATP depletion and Cancer Cell Death following Calcium Electroporation, Relative Effect of Calcium Concentration and Electric Field Strength
Year 2015
Issue 10 (4)
Pages e0122973
Status Published

Conference abstract(s) originating from this mission

Publication IFMBE Proceedings of the 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Publisher Tomaz J, Kramar P (ed), 1st edn. Springer-Verlag, Singapur
Authors Stefania Romeo, Emilie L. Hansen, Stine K. Frandsen, Julie Gehl
Title Electroporation of a Bladder Cancer Cell Line in Presence of Calcium: Efficacy Dependence on Electric Field Strength and Calcium Concentration
Year 2015
Issue 53
Pages 151-154
Status Published

Oral presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period 06/09 - 10/09/2015
Authors Stefania Romeo, Emilie L. Hansen, Stine K. Frandsen, Julie Gehl
Title Electroporation of a Bladder Cancer Cell Line in Presence of Calcium: Efficacy Dependence on Electric Field Strength and Calcium Concentration

 


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