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Evaluation of novel electrode for the purpose of vaccination and skin gene therapy

STSM by Špela Kos, PhD Student

Period of mission: from 01/03/2015 to 31/05/2015

Home institution: Institute of Oncology Ljubljana, Ljubljana, Slovenia

Host institution: Louvain Drug Research Institute (LDRI), Catholic University of Louvain, Brussels, Belgium

Skin gene electrotransfer (GET) is a safe and efficient method to deliver the genes to skin tissue. Its transfection efficiency depends on different parameters, including the type of electrodes for optimal electric pulses application. So far, different electrodes have been used to deliver the genes to skin, such as plate electrodes, needle electrodes, as well as novel multi-array electrodes (MEA). Based on the previous studies, GET with MEA resulted in minimal skin damages with great reduction of muscle contraction. Therefore, the aim of our study carried out during STSM, was to investigate the novel MEA electrodes for the purposes of DNA vaccination and gene delivery to skin. The transfection efficiency obtained with MAE as compared to plate electrodes was first evaluated in vivo on a C57/BL6 mouse model. Efficiency of GET with MEA was evaluated by following luciferase gene expression after intradermal injection of plasmid DNA (pDNA) and by characterizing the immune response after immunisation with model therapeutic pDNA coding for ovalbumin (OVA).

The results indicate that novel multi-array electrodes promoted higher and longer duration of gene expression compared to luciferase gene delivery by plate electrode. Further on, the results indicate on strong humoral and cellular immune response after MEA application, with a significant delay in tumour growth after immunization. 

The results gained during STSM proved the effectiveness of MEA electrodes for skin gene delivery and characterizes them as a promising tool for gene therapy and vaccinations.

Poster presentation(s) originating from this mission

Event PhD students' day, organized by Louvain Drug Research Institute
Location Brussels/Belgium
Period

21/05/2015

Authors Spela Kos, Tanja Blagus, Kevin Vanvarenberg, Maja Cemazar, Véronique Préat, Gregor Sersa, Gaëlle Vandermeulen
Title Utilization of gene electrotransfer for delivery of plasmid DNA to the mouse skin

 


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