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Pulsed Electric Field for protein release from microalgae

STSM by Richard Postma, PhD Student

Period of mission: from 18/05/2014 to 25/05/2014

Home institution: Wageningen University, Wageningen, The Netherlands

Host institution: ProdAl Scarl, Fisciano, Italy

Recently the cultivation and refinery of microalgae biomass has stimulated intensive research due to its relative high content of more than 50% of valuable cell components, like proteins, lipids, polysaccharides, and pigments. Besides, the areal productivity is much higher compared to other agricultural plants. The aim of this STSM was to explore the possibilities to use Pulsed Electric Field (PEF) for extraction of water soluble proteins from microalgae. The microalgae Chlorella vulgaris was cultivated in a 12 L photobioreactor at Wageningen University in Netherlands under both nitrogen replete (N+) and nitrogen deplete (N-) conditions. By means of centrifugation the algal harvest was concentrated and then stabilized as freeze dried. At ProdAl Scarl, Fisciano, Italy the microalgal suspensions were prepared at 25 g/L for both the N+ and N- algae by resuspending the freeze dried material in tap water and rehydrated at 4 °C overnight. Five PEF experiments were performed at different field strengths (20 and 30 kV/cm) and specific energy consumptions (50 and 100 kJ/kg) on both the N+ and N- biomass. The water soluble protein concentration in the supernatant was measured. Unfortunately, the freeze drying caused the cells to break, releasing the water soluble proteins in the supernatant after resuspension. Therefore no significant increase of protein was obtained after PEF. This leads to the conclusion that PEF can only be performed on fresh biomass.

Publication(s) originating from this mission

Journal Bioresource Technology
Impact Factor (according to ISI Thomson) 4,494
Authors PR Postma, G Pataro, M Capitoli, MJ Barbosa, MHM Eppink, RH Wijffels, G Olivieri, G Ferrari
Title Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined Pulsed Electric Field-Temperature treatment
Year 2015
Issue ?
Pages ?
Status Submitted - to be updated

Oral presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period 06/09 - 10/09/2015
Authors Richard Postma, Giuseppe Olivieri, Maria Barbosa, Rene Wijffels, Michel Eppink, M Capitoli, Gianpiero Pataro, Giovanna Ferrari
Title Effect of temperature and energy input on PEF processing of the green microalgae Chlorella vulgaris

 


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