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Evaluation of dielectrophoretic behavior of electroporated cells

STSM by Mihaela Moisescu, Early-Stage Researcher

Period of mission: from 15/03/2015 to 09/04/2015

Home institution: Biophysics and Cell Biotechnology Dept., Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Host institution: UMR 8203 CNRS, University Paris Sud and Gustave Roussy Institute, Villejuif, France

Two types of dielectrophoretic (DEP)-controlled movements are experimentally observed: positive-DEP and negative-DEP (as cell moves respectively, toward or against a high intensity region of the field), each of them occurring in a characteristic range of AC electric field frequencies. By a proper selection of the DEP field frequency, separation of cell categories in a mixture or evaluation of cell dielectric properties becomes possible.

The experimental work carried out during this STSM exploited the 3D DEPtech system recently available in the Host institution by determining the DEP spectra of electroporated cells and correlating the dielectric parameters of the cells with the conditions of electroporation.

By monitoring the changes in optical density of a cell suspension subject to DEP field, the device allowed obtaining a 20-points DEP spectrum since it works with 20 wells energized by different AC field frequencies. This system allowed rapid analysis (within tens of seconds) of thousands of cells simultaneously. DEP spectra of electroporated cells has been acquired as function of exterior medium conductivity (400, 100 and 10 μS/cm) and of time after pulses’ delivery (2 to 30 min).

The single shell dielectric model for a cell in suspension is implemented into the analysis module of the 3D DEPtech system. When a measured DEP spectrum has been analyzed, certain parameters had to be declared, like: exterior medium conductivity and permittivity and cell radius (measured on the actual cell suspension used in the experiments). Dielectric parameters like: specific membrane capacitance and conductance, or cytosol conductivity for electroporated cells could be thus calculated and their evolution in time evaluated.

Poster presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period

06/09 - 10/09/2015

Authors Violeta Liuba Calin, Mihaela Georgeta Moisescu, Irina Grigorescu, Liviu Dimitriu and Tudor Savopol
Title Investigation of optical and mechanical characteristics of electroporated cells using optical tweezers

 

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period

06/09 - 10/09/2015

Authors Mihaela G. Moisescu, Mihai Radu, Isabelle Leray, Minodora M. Iordache, Tudor Savopol and Lluis M. Mir
Title Chemical membrane permeabilisation provides insights on the membranes biophysical parameters changes during cell electroporation

 


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