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Effect of hyaluronidase on early inflammatory events induced by electrotransfer in mouse skin compared to skeletal muscle

STSM by Lise Pasquet, Early-Stage Researcher

Period of mission: from 05/05/2014 to 30/05/2014

Home institution: IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse, France

Host institution: CNR-IFT (Institute of Translational Pharmacology) and University Campus Bio-Medico, Rome, Italy

In this STSM we addressed if the use of Hyaluronidase, an enzyme responsible for the degradation of the extracellular matrix, could increase (i) the accessibility of plasmid DNA to target cells in skin submitted to EGT protocols and (ii) the resulted immune inflammation, as it was demonstrated in muscles. Balb/c mice were injected intra-dermally or intramuscularly (both posterior legs) with Hyaluronidase and a combination of IL-12 and Tomato plasmids. The animals underwent to the following electropermeabilisation parameters applied with BTX ECM830 Electroporator (Harvard Apparatus): 4 pulses of 1000V/cm, 100µs + 4 pulses of 250V/cm, 20ms at 1Hz of frequency with 10 mm diameter plate electrodes at 0.1 cm of distance for skin; 10 pulses of 175V/cm, 20ms, 1Hz with 10 mm diameter plate electrodes at 0.4 cm of distance for muscles. Skins, muscles and spleens were collected at 3h, 7 days and 14 days after the treatment. The sample prepared for histological analysis revealed the presence of immune infiltration only in muscles treated with hyaluronidase and EGT, not in skin. By ELISA quantification of cytokine production in tissue, we observed a higher production of inflammatory cytokines IL-6 and TNF-?, from 3h after the treatment both in muscles and in skin.These first results confirm that Hyaluronidase increases the inflammatory response induced by EP in muscles but also reveal interestingly the same effect in skin.

Oral presentation(s) originating from this mission

Event Gordon Research Seminar in Bioelectrics
Location Maine/USA
Period

05/07 - 06/07/2014

Authors Lise Pasquet
Title Currative vaccination against melanoma in mice by IL12

Poster presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period

06/09 - 10/09/2015

Authors Muriel Golzio, Lise Pasquet, Justin Teissié, Marie-Pierre Rols and Sophie Chabot
Title Vaccination against melanoma in mice by IL12 electro-geno-immuno-therapy

 


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