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Electroporation of spinach leaf: theoretical and experimental investigation

STSM by Katarzyna Dymek, PhD student

Period of mission: from 01/09/2012 to 30/11/2012

Home institution: Department of Food Technology, Engineering and Nutrition, Lund University, Sweden

Host institution: Faculty of Electrical Engineering, University of Ljubljana, Slovenia

One of the promising applications of reversible electroporation of plant tissue is the improvement of freezing tolerance of spinach leaves after electroporation and vacuum impregnation treatment (Phoon et al. 2008). Spinach leaf possesses heterogeneous internal structure. To investigate the electroporation of such complex structure a numerical model of the leaf cross section was built. The model represents the 3D reconstruction of plant tissues. It is based on the microscopic images of the spinach leaf cross section and it includes four layers: single cell layer of epidermal cells, double cell layer of palisade mesophyll and multi cell layer of spongy mesophyll cells. The air fraction is located in the spongy mesophyll. Cells are surrounded by cell walls, which are connected with each other. Moreover, the model representing the vacuum impregnated leaf was also built. This model does not contain the air fraction, which is removed during the vacuum impregnation process. Mathematical equations describing the electroporation of the cellular membrane are based on DeBruin et al. 1999 and the method for calculating the induced transmembrane voltage is adopted from Pucihar et al. 2009. COMSOL Multiphysics 3.5a (COMSOL AB, Stockholm, Sweden) commercial finite element software package was used to perform the calculations. Models were adjusted and validated by comparison with the experimental data. Measurements of the impedance at 20 Hz-100 kHz frequency range were performed in the Franz cells before and after application of high voltage electric pulse to the samples.

This report is also featured in the February 2013 newsletter available here.

Publication(s) originating from this mission

Journal Innovative Food Science and Emerging Technologies, Special Issue
Impact Factor (according to ISI Thomson) 3,273
Authors Katarzyna Dymek; Lea Rems; Barbara Zorec; Petr Dejmek; Federico Gomez Galindo; Damijan Miklavcic
Title Modeling electroporation of the non-treated and vacuum impregnated heterogeneous tissue of spinach leaves
Year 2015
Issue 29
Pages 55-64
Status Published

Oral presentation(s) originating from this mission

Event XXIII International Symposium on Bioelectrochemistry and Bioenergetics of the Bioelectrochemical Society
Location Malmö, Sweden
Period 14/06 - 18/06/2015
Authors Katarzyna Dymek; Lea Rems; Barbara Zorec; Petr Dejmek; Federico Gomez Galindo; Damijan Miklavcic
Title Modeling electroporation of the non-treated and vacuum impregnated heterogeneous tissue of spinach leaves

 


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