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Electro-induced sensitization of cells

STSM by Janja Dermol, PhD Student

Period of mission: from 18/01/2015 to 20/03/2015

Home institution: University of Ljubljana, Faculty of Electrical Engineering, Ljubljana, Slovenia

Host institution: Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, USA

The aim of my STM was to investigate cell sensitization. Cell sensitization is a phenomenon where increasing the duration of the electroporation treatment, for example by splitting the delivered pulses in two shorter trains is reported to significantly increase the cell sensitivity to subsequent pulses. Uptake of different molecules is increased and cell viability is reduced. The mechanisms for occurrence of cell sensitization are not known yet. The suggested possibilities are cell swelling, formation of reactive oxygen species and calcium influx. In this STSM I investigated the connection between cell permeabilization and sensitization. Experiments were performed on human monocyte cell line U937. Pulses were delivered either all in one train or they were split in two trains with a 5 min delay between them. Stable cell sensitization was obtained in a wide range of permeabilizing E-fields. With higher E-fields applied the cell sensitization effect was larger. Cell survival was up to twofold reduced when the same electrical parameters were applied but the second train was delayed for 5 min. In addition it was established that when the delay between the trains is 15 seconds there is already cell sensitization present. Our results are important for clinical applications where lower number of pulses, lower voltages and larger distance between the electrodes can be used if the delivered pulses are split in two parts with a short delay between them.

Poster presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period

06/09 - 10/09/2015

Authors Claudia Muratori, Janja Dermol, Shu Xiao, Andrei Pakhomov and Olga Pakhomova
Title The time course of electroporation-induced sensitization

 

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period

06/09 - 10/09/2015

Authors Janja Dermol, Olga N. Pakhomova, Shu Xiao, Andrei G. Pakhomov and Damijan Miklavčič
Title Cell sensitization is induced by a wide range od permeabilizing electric fields

 


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