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Development of a Matlab-based interface for an optical tweezers system dedicated to electroporated cells

STSM by Irina Grigorescu, MSc Student

Period of mission: from 12/10/2014 to 25/10/2014

Home institution: Carol Davila University of Medicine and Pharmacy, Bucharest, Romania

Host institution: Institut de Pharmacologie et de Biologie Structurale, Toulouse, France

The optical tweezers are a scientific instrument which uses a highly focused laser to provide an attractive or repulsive force to physically move or hold microscopic dielectric objects. I am currently working in performing measurements of the power spectrum of such a device dedicated to studies on electroporated cells. The purpose of this STSM was to develop Matlab software application for acquiring and analyzing the data given by the optical tweezers system and to add features to an existing program. The current software is rigid in parameters outcome and insufficient in providing relevant data since the status of the electroporated cells is changing rapidly in time. The laboratory that hosted me at IPBS is experimenting and using an optical kit similar to the one in Bucharest. Exploiting their experience on correct adjustment of both hard and software components of the device, I gained knowledge in terms of adapting the Matlab interface specialized in controlling the optical tweezers system when single-cell electroporation studies are performed and I applied it when the calibration routines were run.

Poster presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period

06/09 - 10/09/2015

Authors Violeta Liuba Calin, Mihaela Georgeta Moisescu, Irina Grigorescu, Liviu Dimitriu and Tudor Savopol
Title Investigation of optical and mechanical characteristics of electroporated cells using optical tweezers

 


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