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Inactivation of bacterial spores by electric arcs

STSM by Flavien Pillet, Early-Stage Researcher

Period of mission: from 12/05/2014 to 31/05/2014

Home institution: IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse, France

Host institution: University of Ljubljana, Ljubljana, Slovenia

Bacterial spores are involved in the major part of food contamination and also responsible of grave diseases like Bacillus anthracis which is the etiologic agent of anthrax. The lethality of spores is increased by their highly resistance to chemical, thermal and physical stresses. The inactivation of spores raises a grave issue, particularly because non-thermal inactivation like PEF is inefficient. The purpose of this STSM was to use electric arcs for spore inactivation, by using the system developed by Igor Marjanovic and Tadej Kotnik (Bioelectrochemistry, 2013). This device was previously used for bacteria inactivation in vegetative form. However, electric arcs were not still described for spore inactivation. During this STSM the efficiency of spore inactivation in solid and liquid medium were evaluated according to different parameters (voltage, time and number of electric arc). In liquid, spore inactivation was not observed. By contrast, a high level of inactivation was measured in solid medium. With 1 electric spark at 5 kV during 20 µS, an inactivation rate more than 3 log10 was deduced. The phenomenons responsible of inactivation are probably various, like shock wave, ionization, UV radiation etc. Finally these results are particularly promising for decontamination of solid food and a better understood of phenomena involved would be suitable for improving the technique.

Poster presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period

06/09 - 10/09/2015

Authors Matej Reberšek, Igor Marjanovič, Samo Beguš, Flavien Pillet, Marie-Pierre Rols, Damijan Miklavčič and Tadej Kotnik
Title Emulating Exposures of Biological Samples to Lightning Strokes

 


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