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Improving extraction of pigments from microalgae by pulsed electric fields

STSM by Elisa Luengo, Researcher

Period of mission: from 08/07/2013 to 31/07/2013

Home institution: University of Zaragoza, Zaragoza, Spain

Host institution: Institut of Pharmacology and Structural Biology (IPBS) - CNRS, Toulouse, France

Although several studies have demonstrated that electropermeabilization of cells for improving extraction can be achieved with trains of electrical pulses with duration in the range of milliseconds or in the range of microseconds; at the moment, their relative efficacy has not been directly compared. The influence of different protocols of application of pulses, milliseconds and microseconds, on the extraction of pigments from the microalgae Chlorella vulgaris was investigated. Moreover, these studies were combined with observations and measurements performed by microscopy in order to evaluate the PEF treatment effect on C. vulgaris. The application of a PEF treatment of 4.5kV/cm and 60 ms (194 kJ/kg) with a pulse duration between 1 and 3 ms was enough to achieve the maximum carotenoid extraction rate, whereas when C. vulgaris cells were treated with pulses of 3 μs the maximum extraction rate was achieved with a PEF treatment of 20 kV/cm and 150 µs (198 kJ/kg). Batch as well as flow processes were used for extraction. Therefore, in spite of the different pulse duration range, similar energies were needed to achieve the maximum extraction yield. AFM force-volume assay revealed that the application of a PEF treatment of 4.5 kV/cm and 60 ms to C. vulgaris cells induced an increment by 3 times in the stiffness of the envelopes compared with the untreated ones. TEM observations showed a less defined cell wall of the treated cells. However, no differences in the surface were observed between the PEF treated and untreated C. vulgaris cells when they were observed under the SEM.

Publication(s) originating from this mission

Journal Journal of Membrane Biology
Impact Factor (according to ISI Thomson) 2,457
Authors Elisa Luengo, Juan Manuel Martínez, Mathilde Coustets, Ignacio Álvarez, Justin Teissié, Marie-Pierre Rols, Javier Raso
Title A comparative study on the effects of millisecond- and microsecond-pulsed electric field treatments on the permeabilization and extraction of pigments from Chlorella vulgaris
Year 2015
Issue 248
Pages 883-891
Status Published

 


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