print small

Participating Countries:

Algeria

Argentina

Australia

Austria

Belgium

Bosnia and Herzegovina

Bulgaria

Croatia

Czech Republic

Denmark

Finland

France

FYR of Macedonia

Germany

Greece

Iceland

Ireland

Israel

Italy

Lithuania

Morocco

Netherlands

New Zealand

Poland

Portugal

Romania

Russian Federation

Serbia

Slovenia

Spain

Sweden

Switzerland

Turkey

Ukraine

United Kingdom

United States

Member area provided by LTFE
COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST

Nanosecond pulsed electric fields on liposomes for potential drug delivery applications

STSM by Elena Della Valle, PhD Student

Period of mission: from 09/03/2015 to 09/05/2015

Home institution: Department of Information Engineering, Electronics and Telecommunications, “Sapienza” University of Rome, Italy

Host institution: UMR 8203 CNRS, Univ Paris-Sud and Gustave Roussy, Villejuif, France

The use of nanosecond pulsed electric fields (nsPEF) for potential drug delivery applications is one of the most promising techniques which could allow a controlled release of drugs encapsulated in nano-carriers (i.e.liposomes) by the application of an electrical stimulus. The aim of this STSM was to analyze the capabilities of liposomes to mediate drug delivery through cell membrane under nsPEF exposure without causing any damage to the biological cells. With the microdosimetric models, different thresholds were obtained in terms of the external electric field necessary for the poration of the liposome and cell membranes. However the buffer and the inner liposome conductivity can influence these threshold values; moreover the liposome diameter impacts since the largest liposomes in our study (400 nm of diameter) are electroporated with lower electric field amplitudes than the smaller liposomes.

An experimental activity was carried out in order to verify the feasibility of liposome poration with the electric field intensity predicted by the theoretical models. A field amplitude between 3 and 4 MV/m was applied to the DSPC vesicles containing two different populations of liposomes loaded with a fluorescent dye. The fluorescence value was calculated for the exposed and not exposed samples but only a slightly difference was appreciated and no liposome electroporation occurred. Further experiments will be necessary in order to reach the best conditions in terms of liposome dimension and conductivity values to obtain the liposome membrane poration.

Publication(s) originating from this mission

Journal Journal of Membrane Biology, Special Issue
Impact Factor (according to ISI Thomson) 2,174
Authors Agnese Denzi, Elena della Valle, Francesca Apollonio, Marie Breton, Lluis M. Mir, Micaela Liberti
Title Exploring the Applicability of Nano-poration for Remote Control in Smart Drug Delivery Systems
Year 2015
Issue ?
Pages ?
Status Submitted - to be updated

 


Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

Related sites: