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Contribution of Electrochemical Phenomena to the Electroporation Current of Cell Suspension

STSM by Djamel Eddine Chafai, PhD Student

Period of mission: from 08/07/2013 to 30/09/2013

Home institution: APELEC Laboratory, Djillali Liabes University, Sidi Bel Abbes, Algeria

Host institution: Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia

Electroporation of cells is being successfully used in biology, biotechnology and medicine. Practical problems still arise in electroporation of cells in suspension. For example, determination of cell electroporation is still demanding and time consuming task. Also electric pulses cause contamination of the solution by the metal released from the electrodes and create local enhancements of electric field, leading to the occurrence of electrochemical reactions at the electrode/electrolyte interface. Therefore, an estimation of the current due to electrode/electrolyte interface phenomena is of a great interest to determine and evaluate its contribution to the total electroporation current. The goal of the STSM was to study the contribution of the current due to electrochemical phenomena (electrode/electrolyte interface) to the total electroporation current of cell suspension. The study was performed with and without cells in cuvette made of aluminum parallel plate electrodes. Electrical impedance spectroscopy was used before and after the delivery of electric pulses in different solutions of low and high conductivity. Voltage-current measurements were performed in both cases without and with cells. The study yielded to determine the dominant conduction phenomenon of the electrode/electrolyte interface exposed to pulsed electric field in free medium of cells. In presence of cells in low conductive medium, the contribution of electrode/electrolyte interface phenomena (Electrochemical reactions) is less pronounced compared to the intracellular ionic flux when the cell membrane is permeabilized at high electric field.

Publication(s) originating from this mission

Journal Bioelectrochemistry
Impact Factor (according to ISI Thomson) 4,172
Authors Chafai DE, Mehle A, Tilmatine A, Maouche B, Miklavčič D
Title Assessment of the electrochemical effects of pulsed electric fields in a biological cell suspension
Year 2015
Issue 106
Pages 249-257
Status Published

 


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