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Electrochemical properties of cell-mimetic lipid membranes

STSM by Denitsa Mitkova Brankova, PhD Student

Period of mission: from 02/03/2014 to 01/04/2014

Home institution: Institute of Solid State Physics - Bulgarian Academy of Sciences, Sofia, Bulgaria

Host institution: Max Planck Institute of Colloids and Interfaces, Science Park Golm, Potsdam, Germany

During the STSM, we performed experiments for the measurement of important physical properties of lipid membranes, namely their edge tension and electrical capacitance. The edge tension of phosphatidylcholine membranes was studied by electroporation of giant lipid vesicles (GUVs) at identical sugar solutions inside and outside the vesicles. The effect of sucrose in the concentration range from 0 to 400 mM was explored. Pores in the membranes were induced by applying 30-40 V / 5 ms electrical pulses. Asymmetric conditions were also studied allowing for better visualization of the pore. The closure time of the pore is related to the edge tension of the membrane. The experimental results showed a difference between the time closure of the pore in membranes in symmetric and asymmetric solutions. The capacitance of lipid bilayers was measured by electrodeformation of GUVs exposed to AC fields in the kHz frequency range. The GUVs were in aqueous solutions of sucrose at concentrations between 50 and 400 mM with and without 0.1 mM of sodium chloride. The prolate-oblate transition frequency of GUVs is related to the capacitance of lipid bilayers. The forthcoming analysis of the accumulated data will provide a quantitative evaluation of the edge tension and the capacitance of the studied lipid membranes. Our results will help answering the question whether sugar molecules that are dissolved in the aqueous phase exert any effect on the studied physical properties of lipid membranes.

Poster presentation(s) originating from this mission

Event 18th International School on Condensed Matter Physics
Location Varna/Bulgaria
Period

01/09 - 06/09/2014

Authors Denitsa Mitkova, Rumiana Dimova, Victoria Vitkova
Title Investigation of the electrochemical properties of cell-mimetic membranes using electrodeformation and electroporation of lipid vesicles

 

Event Workshop organized by REGPOT-2012-2013-1 NMP INERA EU project
Location Varna/Bulgaria
Period

04/09 - 06/09/2014

Authors Denitsa Mitkova, Rumiana Dimova, Victoria Vitkova
Title Electrochemical properties of cell-mimetic lipid membranes obtained in cells consisting of indium-tin oxide coated plates and polymer spacers

 


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