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Cancer Cell Electrorotation

STSM by Claudia Irene Trainito, PhD Student

Period of mission: from 01/03/2015 to 23/03/2015

Home institution: École Normale Supérieure, Cachan, France

Host institution: Virginia Tech – Wake Forest University School of Biomedical Engineering and Science, Blacksburg, Virginia

Thanks to the support of the COST Action a collaborative project on the characterization of cancer cells at different stages of the illness has been carried on between two research teams: BIOMIS at ENS Cachan (Institut d’Alembert, CNRS UMR8029) and the Bioelectromechanical Systems Laboratory of Virginia Tech-Wake Forest University School of Biomedical Engineering and Science, both specialized on the investigation of the interaction between cells and electric field.

Identification and separation of cells within a complex sample are of prime importance for the cancer diagnosis and treatment.  This project used a combined dielectrophoresis (DEP) and electrorotation (ROT) technique to elucidate the phenotypic differences of sequentially staged cancer cells. First the dielectrophoresis force is applied for cell trapping, and then a rotating electric field through a sample medium is induced. Undergo those two solicitations, the cell experiences a rotational movements and the analysis of its rotational velocity gives as results the dielectric properties such as the permittivity and the conductivity of both membrane and cytoplasm.  Dielectric properties represent electro-physiological traits intrinsic to a cell which are both dependent upon structures both on/within the cell.  Once the electro-physiological fingerprint of the cells is determined, this method can be used as a tool to distinguish malignant cells from healthy ones and determine the patient’s stage of cancer.

Oral presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period 06/09 - 10/09/2015
Authors Claudia Irene Trainito, Emilie Bayart, Emilie Bisceglia, Frederic Subra, Olivier Français, Bruno Le Pioufle
Title Electrorotation as a versatile tool to estimate dielectric properties of multiscale biological samples: from single cell to spheroid analysis

 


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