print small

Participating Countries:

Algeria

Argentina

Australia

Austria

Belgium

Bosnia and Herzegovina

Bulgaria

Croatia

Czech Republic

Denmark

Finland

France

FYR of Macedonia

Germany

Greece

Iceland

Ireland

Israel

Italy

Lithuania

Morocco

Netherlands

New Zealand

Poland

Portugal

Romania

Russian Federation

Serbia

Slovenia

Spain

Sweden

Switzerland

Turkey

Ukraine

United Kingdom

United States

Member area provided by LTFE
COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST

Skin electroporation for transdermal drug delivery - numerical models of in vitro experiments

Reciprocal STSM by Barbara Zorec, PhD Student

Period of mission: from 06/10/2013 to 30/10/2013

Home institution: University of Ljubljana, Ljubljana, Slovenia

Host institution: University of Canterbury, Christchurch, New Zealand

Electroporation as an active enhancement method for dermal and transdermal drug delivery has been used before, however, the possibilities for further improvement of the method are far from being exhausted. Our in vitro experimental results have shown that longer LV pulses significantly increase the transport of calcein through dermatomed pig skin, while short HV pulses alone result in negligible total calcein transdermal transport. Surprisingly, when the long LV pulses are preceded by short duration HV pulses, the total transport is reduced significantly. In order to make sense of these results, theoretical analysis of the underlying mechanisms was needed. Our main focus during the STSM was oriented towards theoretical comparison of different combinations of square wave short high voltage (HV) and longer low voltage (LV) electroporation pulses in order to understand fundamental physics during electroporation. The theoretical model shows that HV pulses alter the structure of the stratum corneum in such a way that when the LV pulses are applied, insufficient thermal energy is generated to initiate the expansion of transport pathways into larger local transport regions (LTRs). Together, the experimental results and theoretical predictions show that the total pulse energy alone cannot account for total solute transport: that the order and the type of pulses administered must also be considered. During the STSM we also upgraded the description of the thermodynamics underlying the LTR evolution with a simplified analytical model that shows directly how the heating time required to initiate LTR evolution is dependent upon the pre-existing pathway size and density.

Publication(s) originating from this mission

Journal Mathematical Biosciences 
Impact Factor (according to ISI Thomson) 1,303
Authors Sid Becker, Barbara Zorec, Damijan Miklavcic, Natasa Pavselj
Title Transdermal transport pathway creation: electroporation pulse order
Year 2014
Issue 257
Pages 60-68
Status Published

Oral presentation(s) originating from this mission

Event 3rd International Conference on Computational Methods for Thermal Problems (ThermaComp2014)
Location Lake Bled/Slovenia
Period 02/06 - 04/06/2014
Authors Barbara Zorec, ??? (to be updated)
Title Computational modeling of skin electroporation: reconciliation between experimental observations and anticipated physics

 

Event 23rd International Symposium on Transport Phenomena
Location Auckland/New Zeeland
Period 19/11 - 22/11/2012
Authors Sid Becker, Barbara Zorec, Nataša Pavšelj, Damijan Miklavčič
Title Transport Modeling of Skin Electroporation and the Thermal Behavior of the Stratum Corneum

Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

Related sites: