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Modification of Franz diffusion cell experimental setup for physical enhancement methods

STSM by Barbara Zorec, PhD Student

Period of mission: from 05/10/2014 to 08/11/2014

Home institution: Iskra Medical d.o.o., Ljubljana, Slovenia

Host institution: University of Canterbury, Department of Mechanical Engineering, Christchurch, New Zealand

Franz diffusion cells represent the gold standard in skin passive diffusion studies, but are not the ideal in vitro setup to be used in transdermal delivery studies with electroporation and other physical enhancement methods. Due to the geometric constraints, delivering electric pulses to the skin inside the diffusion cell is challenging. The electric pulses are delivered through the electrodes placed in the donor and the receiver solution, so most of the delivered voltage rests in the buffer itself and only a small part on the skin. For ultrasound testing the donor compartment of Franz diffusion cells system is way too small. We aimed to modify Franz diffusion cells, to be better suited for experiments with physical enhancement methods of transdermal molecular delivery. We considered parameters that we needed to improve: Ultrasound donor chamber designed especially for sonoporation (minimizes acoustic interference) and 3D printed strain clamp allows for seamless integration of standard cell receiver with novel donor chamber. With this in our mind we developed a modified diffusion cell, which addressed the problems we have outlined. Donor compartment is much larger and with that suitable for other enhancement techniques. The material choices for the donor chamber and clamp were made according to just little reflections that are expected. After the preliminary experiments we confirm that the results of our work have delivered a newly designed donor chamber and clamp for Franz diffusion cells to be suitable for side-by-side electroporation and sonoporation.

Publication(s) originating from this mission

Journal Journal of Biomechanical Engineering
Impact Factor (according to ISI Thomson) 1,780
Authors Nataša Pavšelj, Barbara Zorec, Damijan Miklavčič, Sid Becker
Title Experimental Factors to be Consideredin Electroporation-Mediated Transdermal Diffusion Experiments
Year 2015
Issue ?
Pages ?
Status Accepted for publication - to be updated 

Oral presentation(s) originating from this mission

Event 3rd International Conference on Computational Methods for Thermal Problems (ThermaComp2014)
Location Lake Bled/Slovenia
Period 02/06 - 04/06/2014
Authors Barbara Zorec, ??? (to be updated)
Title Computational modeling of skin electroporation: reconciliation between experimental observations and anticipated physics

 

Event 23rd International Symposium on Transport Phenomena
Location Auckland/New Zeeland
Period 19/11 - 22/11/2012
Authors Sid Becker, Barbara Zorec, Nataša Pavšelj, Damijan Miklavčič
Title Transport Modeling of Skin Electroporation and the Thermal Behavior of the Stratum Corneum

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