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Molecular dynamics study of the voltage sensitive dye ANNINE-6

STSM by Aude Silve, Researcher

Period of mission: from 12/05/2013 to 26/05/2013

Home institution: IHM KIT, Karlsruhe, Germany

Host institution: University of Groningen, Groningen, Netherlands

The Bioelectrics group of the Institute for Pulsed Power and Microwave Technology at KIT possesses a setup designed to measure transmembrane voltage modulation during exposure of cells to nanosecond pulsed electric fields. The measurement is based on the use of a voltage-sensitive fluorescent dye (VSD, ANNINE-6) which incorporates in the membrane of cells. Providing quantitative measurement of transmembrane voltage requires however a calibration of the ANNINE-6 molecule. The STSM was performed with the Molecular Dynamics group of the University of Groningen. We addressed the question of the calibration of ANNINE-6 by means of computation. Molecular dynamic simulations combined with a quantum mechanics (QM) approach were used to provide information for the calibration, especially on the nanosecond timescale. The position, orientation and spectral shift of the ANNINE-6 molecule in a POPC bilayer has been analysed for positive and negative electric field with an absolute value of 0.1 V/nm. Preliminary results show a clear asymmetry in the behaviour of the dye that could explain some asymmetry in the calibration. Additionally, time course of the rearrangement of ANNINE-6 molecule and of its environment has been studied. The preliminary results indicate rearrangements on the nanosecond timescale. This will be further investigated and will inform us on the possibility to transpose long time scale calibration to short time scale measurements.

Oral presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period 06/09 - 10/09/2015
Authors Aude Silve, Clair Poignard, Martin Sack, Ralf Straessner and Wolfgang Frey
Title Study of transmembrane voltage kinetics during 100 μs pulse using voltage sensitive dyes

Poster presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period

06/09 - 10/09/2015

Authors Aude Silve, Isabelle Leray, Clair Poignard and Lluis M Mir
Title Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses

 


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