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Micro-gap electrodes for electropermeabilization

STSM by Antoine Azan, PhD Student

Period of mission: from 31/03/2014 to 04/04/2014

Home institution: UMR 8203 of the CNRS and University Paris-Sud, Institut Gustave Roussy, Villejuif, France

Host institution: IHM KIT, Karlsruhe, Germany

Although known for decades, the underlying mechanisms of cell electropermeabilization are still not fully understood. One of the most credible hypotheses is the chemical alteration of the membrane phospholipids due to the pulsed electric field. Coherent Anti-stokes Raman Scattering (CARS) microscopy is a powerful tool to acquire image at a single vibrational frequency associated to vibrational mode of chemical bonds. It allows a biochemical decomposition of the sample with a high time resolution (3ns). At UMR8203, a CARS microscope can be coupled with a pulsed generator. The purpose of the STSM is to manufacture micro-gap electrodes on microscope slide in order to be able to pulse the biological sample under the CARS microscope. The laboratory IHM in Karlsruhe Institute of Technology has the technical skills and the background to glue electrodes on substrate with a thin glue layer in order to provide a homogenous field. During the STSM, we successfully manufactured micro-gap electrodes on two different substrates: microscope slide and optical filter. The process is artisanal and has to be performed really carefully due to the fragility of the substrates. With preliminary tests, the process has been optimized for each type of substrate and controlled at several checkpoints. The optical coating has to be checked under the CARS microscope at UMR8203 in order to be sure that it is not damaged. The manufactured gap is really an improvement for the CARS microscope and provides a lot opportunity to work on many topics and investigate the biochemical characterization of electropermeabilization.

Publication(s) originating from this mission

Journal Bioelectrochemistry
Impact Factor (according to ISI Thomson) 4,172
Authors Tomás García-Sánchez, Antoine Azan, Isabelle Leray, Javier Rosell-Ferrer, Ramon Bragós, Lluis M. Mir
Title Interpulse multifrequency electrical impedance measurements during electroporation of adherent differentiated myotubes
Year 2015
Issue 105
Pages 123-135
Status Published

Oral presentation(s) originating from this mission

Event 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
Location Portorož/Slovenia
Period 06/09 - 10/09/2015
Authors Tomas Garcia-Sanchez, Antoine Azan, Isabelle Leray, Javier Rosell-Ferrer, Lluis M. Mir and Ramon Bragos
Title Modeling dynamic electrical impedance spectroscopy measurements on electroporated cells

 


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