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Nanosecond pulses effects on cells: microdosimetry supporting experiments

STSM by Agnese Denzi, PhD student

Period of mission: from 15/04/2013 to 24/05/2013

Home institution: Department of Information Engineering, Electronics and Telecommunications, Sapienza, University of Rome, Rome, Italy

Host institution: UMR 8203 of the CNRS and University Paris-Sud, Institut Gustave Roussy, Villejuif, France

Recently the use of electric pulses always more intense and shorter, allowed the manipulation of intracellular membranes. The aim of the STSM was to analyze the possibility to couple theoretical and experimental aspects in order to understand the electropermeabilization phenomenon for both the outer and the inner membranes of the cells. The main experimental activity was on human adipose mesenchymal stem cells with one 10 nanoseconds pulse with different amplitudes (30 kV/cm-350 kV/cm) to analyze the value of the external electric field necessary to obtain the permeabilization of the plasma and the endoplasmic reticulum (ER) membranes. The experiments were carried out with two different external buffer solutions with (DMEM) and without (SMEM) calcium ion (Ca2+). The first allows to obtain the threshold for the plasma membrane whereas the second one the threshold for the ER membranes. The main result of the host laboratory was the finding that there are different thresholds in terms of the external electric field necessary to electropermeabilize the different membranes of the cells. With the microdosimetric model, different thresholds were obtained in terms of the external electric field for the permeabilization of the membranes. However the dielectric parameters of the organelle can greatly influence these threshold values up to the point that in some conditions the permeabilization for the inner membrane appears with electric field values lower than for the outer one. The microdosimetric model can be useful to understand the particular conditions that determine the different thresholds between the inner and the outer membranes.

Publication(s) originating from this mission

Journal Journal of Membrane Biology
Impact Factor (according to ISI Thomson) 2,174
Authors Agnese Denzi, Caterina Merla, Paola Camilleri, Alessandra Paffi, Guglielmo d’Inzeo, Francesca Apollonio, Micaela Liberti
Title Microdosimetric Study for Nanosecond Pulsed Electric Fields on a Cell Circuit Model with Nucleus
Year 2013
Issue 246
Pages 761-767
Status Published

 

Journal Journal of Membrane Biology, Special Issue
Impact Factor (according to ISI Thomson) 2,174
Authors Agnese Denzi, Elena della Valle, Francesca Apollonio, Marie Breton, Lluis M. Mir, Micaela Liberti
Title Exploring the Applicability of Nano-poration for Remote Control in Smart Drug Delivery Systems
Year 2015
Issue ?
Pages ?
Status Submitted - to be updated

 


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