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22/02/2016 (Added to site)
Author(s): Postma, P. R.; Pataro, G.; Capitoli, M.; Barbosa, M. J.; Wijffels, R. H.; Eppink, M. H.; Olivieri, G.; Ferrari, G.

Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field–temperature treatment

Journal: Bioresource Technology, 203/1 (2016), pp. 80-88
DOI: 10.1016/j.biortech.2015.12.012
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Abstract: The synergistic effect of temperature (25-65°C) and total specific energy input (0.55-1.11kWhkgDW(-1)) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55°C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25-39% carbohydrates and 3-5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55°C. Above 55°C spontaneous cell lysis occurred without PEF. Combined PEF-temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40-45% protein, 48-58% carbohydrates).



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