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19/11/2012 (Added to site)
Author(s): Lee, E. W.; Wong, D.; Tafti, B. A.; Prieto, V.; Totonchy, M.; Hilton, J.; Dry, S.; Cho, S.; Loh, C. T.; Kee, S. T.

Irreversible Electroporation in Eradication of Rabbit VX2 Liver Tumor

Journal: Journal of Vascular and Interventional Radiology, 23/6 (2012), pp. 833-840
DOI: 10.1016/j.jvir.2012.02.017
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Abstract:

Purpose: To show the effectiveness and safety of irreversible electroporation (IRE) in treating large tumor models.

Materials and Methods: VX2 liver tumor implantation was performed in 35 New Zealand White Rabbits. The rabbits were divided into three groups 1 week after implantation. The control group included 15 rabbits; the remaining 20 rabbits were divided into two IRE treatment groups. For the treatment groups, 10 rabbits underwent ablation with a single IRE application (IRE-S group), and 10 rabbits underwent ablation with multiple IRE applications (IRE-M group). Treatments and outcomes were analyzed using ultrasound, contrast-enhanced computed tomography (CT), and immunohistologic staining (hematoxylin and eosin [H&E], P-53, Ki-67, CD30, and vascular endothelial growth factor receptor [VEGFR] staining, and terminal deoxynucleotidyl-transferase–mediated 2’-deoxyuridine 5’-triphosphate [dUTP]–biotin nick-end labeling [TUNEL] assay).

Results: Multiple IRE ablations consistently produced complete cell death in all the animals in the IRE-M group (n  10, IRE ablation time 2.45 minutes  0.3). The results were validated with ultrasound, CT, H&E, Ki-67, P53, and TUNEL assay. A high level of CD30-positive cells were identified in the IRE groups. A sharply demarcated ablation zone with no damage to surrounding vital structures was observed in all IRE-treated tissues. No complications during or after ablation were observed in any of the animals.

Conclusions: The effects of IRE were shown in a large tumor model with single and multiple IRE ablations (IRE-S and IRE-M treatment groups); complete ablation of the tumor was seen in the IRE-M group. These findings successfully show the beneficial effects and safety of IRE in the treatment of tumors and validate its potential as a clinically translatable treatment.



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