print small

Participating Countries:

Algeria

Argentina

Australia

Austria

Belgium

Bosnia and Herzegovina

Bulgaria

Croatia

Czech Republic

Denmark

Finland

France

FYR of Macedonia

Germany

Greece

Iceland

Ireland

Israel

Italy

Lithuania

Morocco

Netherlands

New Zealand

Poland

Portugal

Romania

Russian Federation

Serbia

Slovenia

Spain

Sweden

Switzerland

Turkey

Ukraine

United Kingdom

United States

Member area provided by LTFE
COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST
10/04/2014 (Added to site)
Author(s): Dehez, F.; Delemotte, L.; Kramar, P.; Miklavčič, D.; Tarek, M.

Evidence of Conducting Hydrophobic Nanopores Across Membranes in Response to an Electric Field

Journal: Journal of Physical Chemistry C, 118/13 (2014), pp. 6752–6757
DOI: 10.1021/jp4114865
Request reprint  |  Tell your friend  | 

Abstract: Electroporation, the application of electric fields to alter the permeability of biological membranes, has recently become a clinical tool for the electrochemotherapy treatment of various cancers. Current electroporation theory assumes that the membrane is permeabilized through the formation of conducting hydrophilic pores, stabilized by rearrangement of lipid head groups. Here we have performed molecular dynamics simulations of negatively charged lipid bilayers subject to high transmembrane voltages together with electroporation experiments on planar bilayers. Our data reveal a hitherto unknown electroporation process in which large ion-conducting water columns not stabilized by lipid head groups are formed within the bilayer’s hydrophobic core. The existence of such hydrophobic pores challenges the standard theoretical description of pore creation in lipid membranes. Our findings open a new vista toward fine-tuning of electroporation-based treatments and biotechnical applications, and, in general, for enhancing the import of various substrates in liposomes or cells.



Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

Related sites: