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20/11/2013 (Added to site)
Author(s): Juan L. Vásquez, Julie Gehl, Gregers G. Hermann

Electroporation enhances mitomycin C cytotoxicity on T24 bladder cancer cell line. A potential improvement of intravesical chemotherapy in bladder cancer

Journal: Bioelectrochemistry, 88 (2012), pp 127-133
DOI: 10.1016/j.bioelechem.2012.08.001
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Abstract: Intravesical mitomycin instillation combined with electric pulses is being used experimentally for the treatment of T1 bladder tumors, in patients unfit for surgery. Electroporation may enhance the uptake of chemotherapeutics by permeabilization of cell membranes. We investigated if electroporation improves the cytotoxicity of mitomycin. In two cell lines, T24 (bladder cancer cell line) and DC3F (Chinese hamster fibroblast), exposure to different concentrations of mitomycin (0.01-2000µM) was tested with and without electroporation (6 pulses of 1kV/cm, duration: 99µs, frequency: 1Hz). Cell viability was assessed by colorimetric assay (MTT). For both cell lines, mitomycin's IC_50 was approximately 1000µM in both pulsed and unpulsed cells. On T24 cells, electroporation and mitomycin caused (relative reduction) RR of survival of: 25%, 31% and 29%, by concentrations 0µM, 500µM and 1000µM respectively. For DC3F cells, the RRs of survival were: 28%, 29%, and 33%, by concentrations 0µM, 500µM and 1000µM respectively. In conclusion, electroporation and mitomycin together are about 30% more effective than mitomycin alone. The results help to elucidate the additive effect of mitomycin and electric pulses and support the use of this combination in the treatment of bladder cancer.


Keywords: cancer    cancer treatment    electroporation   

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