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16/08/2013 (Added to site)
Author(s): Yi, J.; Barrow, A. J.; Yu, N.; O'Neill, B. E.

Efficient electroporation of liposomes doped with pore stabilizing nisin

Journal: Journal of Liposome Research, 23/3 (2013), pp. 197-202
DOI: 10.3109/08982104.2013.788024
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Abstract:

Context: Liposomes have a long history as passive and active drug carriers. Recently, a few methods have been realized to control the
release from liposomes, including heating, ultrasound and laser.

Objective: We report on a new approach to drive release from liposomes using electric fields.

Materials and methods: Liposomes were manufactured containing a high concentration of (quenched) 5-6 carboxyfluorescein dye. Nisin, a well-known amphiphilic peptide lantibiotic that works by stabilizing pores formed in cell membranes, was mixed in solution inside or outside the liposomes. The liposomes were then electroporated using a range of voltages, and assayed for increases in fluorescence due to release of dye. Release was measured against positive and negative controls, with positive control release driven by a strong detergent.

Results: Our results demonstrate that the addition of nisin significantly reduces the electric field required to release the contents of liposomes, from 2000 V/m to approximately 200 V/m. This result proves that, in principle, electroporation (EP) of liposomes doped with small amounts of amphiphilic pore stabilizing peptides may be a practical means to drive release of liposomal contents in vivo.

Conclusion: Drug delivery from liposomes doped with amphiphilic peptides using EP is feasible. This technique could be developed into a potent adjuvant to tumor ablation using irreversible EP.



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