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05/05/2014 (Added to site)
Author(s): Rebersek, M.; Miklavčič, D.; Bertacchini, C.; Sack, M.

Cell membrane electroporation – Part 3: The equipment

Journal: IEEE Electrical Insulation Magazine, 30/3 (2014), pp. 8-18
DOI: 10.1109/MEI.2014.6804737
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Abstract: As described in Part 1, a cell membrane can be made permeable to various molecules by carrying out a procedure called electroporation [1]. This procedure is being successfully used in biology, biotechnology, and medicine [2], [3]. It requires electroporators and electrodes. An electroporator generates short HV pulses of specific shape, amplitude, duration, number, and repetition frequency [4], and the pulses are applied to the target cells or load through the electrodes [5]. The energy delivered to the load is governed by the number of pulses and the pulse voltage, current, and duration. In biomedical applications that energy can be several joules; in biotechnology, where electroporation is used for treatment of agricultural products and water, it can be several kilojoules.



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