print small

Participating Countries:

Algeria

Argentina

Australia

Austria

Belgium

Bosnia and Herzegovina

Bulgaria

Croatia

Czech Republic

Denmark

Finland

France

FYR of Macedonia

Germany

Greece

Iceland

Ireland

Israel

Italy

Lithuania

Morocco

Netherlands

New Zealand

Poland

Portugal

Romania

Russian Federation

Serbia

Slovenia

Spain

Sweden

Switzerland

Turkey

Ukraine

United Kingdom

United States

Member area provided by LTFE
COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST
22/10/2013 (Added to site)
Author(s): Gibot, L; Rols, M.-P.

3D Spheroids' Sensitivity to Electric Field Pulses Depends on Their Size

Journal: Journal of Membrane Biology, 246/10 (2013), pp. 745-750
DOI: 10.1007/s00232-013-9535-x
Request reprint  |  Tell your friend  | 

Abstract: Dramatic differences of cells behavior exist between cells cultured under classical 2D monolayers and 3D models, the latter being closer to in vivo responses. Thus, many 3D cell culture models have been developed. Among them, multicellular tumor spheroid appears as a nice and easy-to-handle 3D model based on cell adhesion properties. It is composed of one or several cell types and is widely used to address carcinogenesis, or drugs screening. A few and recent publications report the use of spheroids to investigate electropermeabilization process. We studied the response of spheroids to electrical field pulses (EP) in terms of their age, diameter or formation technique. We found that small human HCT-116 colorectal spheroids are more sensitive to electric field pulses than larger ones. Indeed, the growth of spheroids with a diameter of 300 µm decreased by a factor 2 over 4 days when submitted to EP (8 pulses, lasting 100 µs at a 1,300 V/cm field intensity). Under those electrical conditions, 650 µm spheroids were not affected. These data were the same whatever the formation method (i.e. hanging drop and nonadherent techniques). These observations point out the fact that characteristics of 3D cell models have to be taken into account to avoid biased conclusions of experimental data.


Keywords: 3D    electrochemotherapy    electropermeabilisation    growth    size    spheroid   

Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

Related sites: