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Bosnia and Herzegovina



Czech Republic




FYR of Macedonia










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Russian Federation








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COST is supported by the EU Framework Programme Horizon 2020
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Institute of Biophysics and Biomedical Engineering (IBhBME), Bulg. Acad. Sci.

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acad. G. Bonchev str. Bl21
1113 Sofia

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Brief presentation of the institution

Department “Electroinduced and adhesive properties” is involved in Institute of Biophysics and Biomedical Engineering (IBPhBME) at Bulgarian Academy of Science, Sofia. Head of the department is Prof. Iana Tsoneva. The director of IBPhBME is Prof. Andon Kossev. The institute was established in June 2010 as a union of the former Institute of Biophysics and the former Centre of Biomedical Engineering “Prof. Ivan Daskalov”. The department works in collaboration with the department Analysis and Processing of Biomedical Signals and Data with a head Prof. Ivailo Christov at the same Institute. The other collaborators of our department are Prof. E. Peycheva – Specialized Hospital for Active Treatment of Oncology, Sofia and Assoc. Prof. E. Borisova - Institute of Electronics, Bulgarian Academy of Science, Sofia. The scientific projects are predominantly multidisciplinary and involved biologists, engineers, physicians, and physicists.

Main Electroporation related topics

The main research topics of the department are: Electroporation, electrofusion and electrotransformatiom. Electrotransfer of macromolecules through lipid membranes. Electrical properties of biological membranes. Electrochemotherapy of tumour cells. Cell adhesion and reorganization of cytoskeleton after electrotreatment. Influence of low voltage, low frequency electric field on the adhesive properties of nanostructures (tissue engineering). The design and construction of instrumentations with aim to improve electroporation or achieving higher efficiency of cancer electrochemotherapy.

Main Electroporation related research techniques and equipment

It was developed by the engineering group of the Institute apparatus “Chemopulse – I, II, III and IV”.  In the last version of the electroporator two elements (electroporator and QRS synhronisator) were incorporated in one body. The device for patient current is developed also. The new instrument is with battery supply, enhanced protection against electrical hazards both for patient and the physician,automatically providing for more than 200 shocks with one battery charging. By using the electroporator with QRS synchronization the patients with pacemakers and heard problems can be treated also. Data collection of the pulse parameters and the impedances of treated patients with surface tumors could be used to support the optimization of the voltage shock setting and the evaluation of the procedure effectiveness. The constructed electroporators are small and portable. The electrotreatment is done by 16 biphasic rectangular pulses, 50 + 50 micros duration each, with a 20 micros interval between both phases and a pause of 880 micros between the bipolar pulses, the amplitude of voltage is from 100 - 2200V.


Research topics, techniques and equipment not related to Electroporation

Microscopes Leica, Germany; Computers; Spectra Fluor Plus plate, reader Tecan, Germany: Spectrophotometer Specord 210 plus analytic, Germany; Fluorescence spectrophotometer F 7000, Hitachi, Japan.

Brief information on available facilites

Two laboratories for cell cultures, chemical laboratory with standard techniques


Willingness to accept/host visiting researchers



  • French-Bulgarian Laboratory “Vesicles and Membranes” 1997-2002
    Project type: International
    Funding: 200 000 eur
  • Mechanism of electrical permeabilisation, DFG, with Prof. E. Neumann, Germany, 436-100, 1993-1998 (2 projects).
    Project type: International
    Funding: 10 000 dm
  • Biophysical Mechanism of the effect of electromagnetic field on biological and model membrane, EU project-COST 244, 1994-1996.
    Project type: International
    Funding: 20 000 usd
  • Effect of electric field on macromolecules in biological and model membranes, K-707, 1997-2002
    Project type: National
    Funding: 5 000 bgn
  • Effect of electrical pulses at the interaction and transfer of poly ions through biological membranes, K-411, 1994-1997.
    Project type: National
    Funding: 8 000 bgn
  • Electrotransfer of anticancer drugs and DNA, French-Bulgarian project, (CNRS-BAS), with Dr. M.P. Rols, Toulouse, France, 2006-2007
    Project type: International
    Funding: 5 500 eur
  • Electrotransfer of macromolecules, K-31, 1991-1994
    Project type: National
    Funding: 3 000 bgn
  • Family of apparatus for electrochemotherapy of malignant tumors: laboratory and clinical studies - prototypes and construction- N 5011, 1998-1999.
    Project type: National
    Funding: 40 000 bgn
  • Mechanism of electroporative DNA and oligo transport through cell membranes and lipid bilayers, with Prof. E. Neumann, Germany, DFG 436/113, 1999-2001.
    Project type: International
    Funding: 10 000 dm
  • Transmembrane transfer of drugs and DNA by electrical pulses, K-1303, 2003-2006.
    Project type: National
    Funding: 12 000 bgn
  • Transmembrane transport of anticancer drugs and DNA by electrical pulses, French-Bulgarian project, (CNRS-BAS), with Dr. M.P. Rols, Toulouse, France, 2003-2005.
    Project type: International
    Funding: 5 500 eur
  • Treatment of cancer cells and solid tumors by suitable choice of electrical pulses
    Project type: National
    Funding: 390 000 bgn


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